Coding

Part:BBa_K3930031

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-08)
Revision as of 16:18, 14 October 2021 by ThomasG (Talk | contribs)


Gene coding for the 9-Hydroperoxide lyase Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 18
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1012

Introduction

This sequence codes for the enzyme the 9-Hydroperoxide lyase that transforms the hydroperoxyde forms of linoleic and linolenic acids into 3,6-nonadienal. This sequence is codon optimized for an expression into S.elongatus. The 9-HPL sequence comes from Cucumis melo and is describes in the publication of (Vincenti et al, 2019).


References

  1. Vincenti S, Mariani M, Alberti J-C, Jacopini S, Brunini-Bronzini de Caraffa V, Berti L, Maury J. 2019. Biocatalytic Synthesis of Natural Green Leaf Volatiles Using the Lipoxygenase Metabolic Pathway. Catalysts. 9(10):873. doi:10.3390/catal9100873.
    2.



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