Part:BBa_K3930030

Gene coding for the 9-lipoxygenase Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1406
  Illegal XhoI site found at 1442
  Illegal XhoI site found at 2425
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 342
  Illegal SapI site found at 1417
  

This sequence codes for the enzyme the 9-lipoxygenase that transforms linoleic and linolenic acids into their hydrperoxyde forms. This sequence is codon optimized for an expression into S.elongatus. The 9-LOX sequence comes from Nicotiana benthamiana.

Introduction

This sequence codes for the enzyme the 9-lipoxygenase that transforms linoleic and linolenic acids into their hydrperoxyde forms. This sequence is codon optimized for an expression into S.elongatus. The 9-LOX sequence comes from Nicotiana benthamiana and is describes in the publication of (Vincenti et al, 2019).

References

1. Vincenti S, Mariani M, Alberti J-C, Jacopini S, Brunini-Bronzini de Caraffa V, Berti L, Maury J. 2019. Biocatalytic Synthesis of Natural Green Leaf Volatiles Using the Lipoxygenase Metabolic Pathway. Catalysts. 9(10):873. doi:10.3390/catal9100873.