Coding

Part:BBa_K3868030

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-01)
Revision as of 14:33, 14 October 2021 by Demon (Talk | contribs)

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P5

Promoter of transketolase 

gene.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pYLXP’- Promoter-Nluc plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 493
    Illegal SpeI site found at 956
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 956
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 774
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 493
    Illegal SpeI site found at 956
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 493
    Illegal SpeI site found at 956
    Illegal AgeI site found at 314
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 193
    Illegal BsaI.rc site found at 881
    Illegal SapI.rc site found at 840


[edit]
Categories
Parameters
None