Coding

Part:BBa_K3868033

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-01)
Revision as of 14:30, 14 October 2021 by Demon (Talk | contribs)

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P8

Promoter of pyruvate dehydrogenase E2 component (dihydrolipoamide acetyltransferase) gene.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pYLXP’- Promoter-Nluc plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 209
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 209
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1246
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 209
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 209
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 426


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Categories
Parameters
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