Composite

Part:BBa_K3885202

Designed by: Xue Li   Group: iGEM21_ZJUT-China   (2021-10-10)
Revision as of 13:32, 14 October 2021 by Snow9 (Talk | contribs)


P70-σ28

Under the influence of the P70 promoter, the transcription factor σ28 is produced and activates expression from the P28 promoter


Usage and Biology

p70-σ28 is a significant part of genecircuit where p70 can be regulated by the σ70 factor present in cell-free system. The rest of the parts in the gene circuit are dependent on the expression of the σ28 factor to initiate expression. Recognition sequence from Escherichia coli str. K-12 substr.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 487
    Illegal SapI.rc site found at 512
    Illegal SapI.rc site found at 668


[edit]
Categories
Parameters
None