Coding

Part:BBa_K3927018

Designed by: Chew Chin Wei   Group: iGEM21_NUS_Singapore   (2021-10-13)
Revision as of 12:53, 14 October 2021 by CWChew (Talk | contribs) (→‎Design)


mFA-HBD2

Coding sequence for the human immune protein, human beta defensin 2 fused to the mating factor alpha peptide for secretion in S.cerevisiae

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 85

Description

Usage

Design

Due to problems associated with expressing recombinant lactoferrin in S. cerevisiae, an alternative antimicrobial peptide (AMPs) was required as a candidate biopesticide. After extensive research into literature and consulting with companies exploring the use of AMPs for agriculture use, Beta-defensins were chosen. Vaciome, a Singaporean synbio startup focusing on AMPs for livestock protection, agreed that in theory, defensins could function as robust antifungals. Jonathan Bester, CEO of Vaciome, strongly believed this project had strong potential to impact the future of crop protection and generously donated to a Human Beta Defensin 2 (HBD2) plasmid optimized for P.pastoris.

Characterisation

[edit]
Categories
Parameters
None