DNA

Part:BBa_K3959000

Designed by: Richard Hou   Group: iGEM21_THIS-China   (2021-10-01)
Revision as of 11:18, 14 October 2021 by Richardhou (Talk | contribs)


modified comD

Throughout our research, we found out that the previous team’s sequence for ComD is not very precise and efficient. Therefore, after doing research and revising, we have revised the sequence base on the easiness to express and preciseness of the protein produced.

In addition to that, after our experimentation, we found out the membrane protein ComD is expressed, but can not properly function, which is to detect the CSP. We then did through research about this protein, and found out that different with other membrane protein, it is a protein without signal peptide, which means that it has to rely on the hydrophobic amino acid to be corrected folded and put on to the membrane.

T--THIS-China--ComD aminoacid analysis.png

We then make sure that it is actually the hydrophobic amino acids that help this protein to get on to the membrane, so we analyze the different kinds of amino acids in this comD protein, and find that 43 percent of the amino acids are hydrophobic.

This fact causes the protein to be hard to formed and transfer onto the membrane correctly. Some of the solution we propose will be that by changing the cultivation environment for the bacteria. For example, the temperature, the type to bacteria we use, codon optimization and etc.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 246
    Illegal EcoRI site found at 388
    Illegal PstI site found at 874
    Illegal PstI site found at 1285
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 246
    Illegal EcoRI site found at 388
    Illegal PstI site found at 874
    Illegal PstI site found at 1285
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 246
    Illegal EcoRI site found at 388
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 246
    Illegal EcoRI site found at 388
    Illegal PstI site found at 874
    Illegal PstI site found at 1285
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 246
    Illegal EcoRI site found at 388
    Illegal PstI site found at 874
    Illegal PstI site found at 1285
    Illegal AgeI site found at 282
  • 1000
    COMPATIBLE WITH RFC[1000]


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