Composite

Part:BBa_K3981013

Designed by: Yulin Peng   Group: iGEM21_AHUT_China   (2021-10-14)
Revision as of 09:19, 14 October 2021 by Registry (Talk | contribs)

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pET28a-vgb-pelb-linker-his-linker-her2-linker-ISZ-sTRAIL

This is a hypoxia-inducible promoter plasmid modified by pET28a-his-linker-her2-linker-ISZ-sTRAIL, in order to enable our modified probiotics to further enhance the killing of cancer cells. In the hypoxic microenvironment of cancer cells, probiotics are affected by hypoxic conditions and secrete a fusion protein with her2, which binds to cancer cell receptors to further enhance the killing ability.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 189
    Illegal NgoMIV site found at 199
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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