Coding

Part:BBa_K4012016

Designed by: Jiayi He   Group: iGEM21_AISSU_Union   (2021-10-13)
Revision as of 02:59, 14 October 2021 by Registry (Talk | contribs)

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MsCHI

MsCHI is the coding sequence that codes for Medicago sativa chalcone isomerase in Medicago sativa. It is the Isomerase involved in the flavonoid biosynthesis. In nature, it has highest expression in young root tips. Medicago sativa chalcone isomerase catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin. Optimum pH is 7-8.5 with 4,2',4'-trihydroxychalcone as substrate, at 25 degrees Celsius. In terms of catalytic activity, a chalcone = a flavanone. EC:5.5.1.6 In our project, the sequence will express chalcone isomerase to convert p-Coumaroyl-CoA into naringenin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None