Coding

Part:BBa_K4012014

Designed by: TIANLANG CHEN   Group: iGEM21_AISSU_Union   (2021-10-13)
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DuLAR(Desmodium uncinatum Leucoanthocyanidin reductase)

DuLAR is a coding sequence that codes for leucoanthocyanidin reductase, an oxidoreductase with ligand NADP, in Desmodium uncinatum. In nature, it catalyzes the synthesis of catechin from 3,4-cis-leucocyanidin. Also synthesizes afzelechin and gallocatechin. In terms of ots catalytic activity, (2R,3S)-catechin + H2O + NADP+ = (2R,3S,4S)-leucocyanidin + H+ + NADPH In our project, the sequence is used to express leucoanthocyanidin reductase to convert leucoanthocyanidin into catechin in saccharomyces cerevisiae.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None