Coding

Part:BBa_K4012012

Designed by: TIANLANG CHEN   Group: iGEM21_AISSU_Union   (2021-10-13)
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CrCPR(Catharanthus roseus NADPH--cytochrome P450 reductase sequence)

CrCPR is a coding sequence that codes for NADPH--cytochrome P450 reductase, an oxidoreductase which has FAD, Flavoprotein, FMN, NADP as ligand in Catharanthus roseus. In nature, the enzyme is This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis. It also has NADPH-dependent ferrireductase activity on the plasma membrane. In term of its catalytic activity, NADPH + 2 oxidized [cytochrome P450] = H+ + NADP+ + 2 reduced cytochrome P450 In our project, the sequence is used to amplify the expression of the sequences in lower course.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 211
    Illegal BglII site found at 946
    Illegal BglII site found at 994
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None