Regulatory

Part:BBa_K3927002

Designed by: Chew Chin Wei   Group: iGEM21_NUS_Singapore   (2021-09-29)
Revision as of 23:16, 13 October 2021 by CWChew (Talk | contribs)


3C120-CYC-LacO

This part encodes for a truncated CYCp core promoter with three C120 repeats replacing the native upstream activating sequence, and a lacO sequence downstream of the TATA box.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 203
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 185
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 203
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 203
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

Usage

Design

Characterization

[edit]
Categories
Parameters
None