Coding
Part:BBa_K3853012
Designed by: Peng Luo Group: iGEM21_CPU_CHINA (2021-09-24)
Revision as of 17:09, 13 October 2021 by Lp-tiffany (Talk | contribs)
sfGFP Codon adapted for Pichia pastoris
This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). This part of the amino acid sequence is the same as BBa_I746916.Using the Thermo GeneArt online tool geneoptimize, we optimize the codon of the protein gene sequence to improve its expression in Pichia pastoris.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |