Reporter

Part:BBa_K3962339

Designed by: Siheng Li   Group: iGEM21_Leiden   (2021-10-11)
Revision as of 14:21, 13 October 2021 by SihengLi (Talk | contribs)


J23100 promoter with mCherry for constant expression

J23100 constitutive promoter is ligated to mCherry for the constant expression of fluorescent proteins. In our project, we used this construct as reference to calibrate the inducibility of L-arabinose on pBAD promoter.

File:Measurement-Figure1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None