Regulatory

Part:BBa_K3753003:Design

Designed by: Jiaying Hu   Group: iGEM21_NJTech_China   (2021-10-13)
Revision as of 11:52, 13 October 2021 by Registry (Talk | contribs)

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pTEF2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 490
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TEF2 maps on chromosome II in the yeast Saccharomyces cerevisiae. pTEF(TEF2) was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The amplified product is a 1000bp sequence upstream of the TEF2 coding sequence, which is a putative TEF2 promoter.


Source

Saccharomyces cerevisiae

References