Part:BBa_K313010

loading sequence of RNA phage MS2

This sequence is necessary for loading of RNA into the capsid of RNA phage MS2. The coat proteins of phage MS2 is known to aggregate around this sequence.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_MS2 Phage MS2] assay page.

We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression. Group: SYSU-CHINA 2021 Author: Zhao bingnan Summary: the 2D and 3D sturcture and application of MS2 aptamer resource:2018_Book_VirusProteinAndNucleoproteinCo,Organization of Intracellular Reactions with Rationally Designed RNA Assemblies Uploads: MS2 aptamer on the circRNA scaffold.png;SYSU-CHINA circRNA-1.png;SYSU-CHINA circRNA-2.png;The principle of spite EGFP.png;MS2 binding mechanism.png

Applications of BBa_K313010

We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.

2D stucture

UNIQ83950070b5c06cdf-partinfo-00000001-QINU UNIQ83950070b5c06cdf-partinfo-00000002-QINU
We use RNAfold and StructureEditor to predict 2D structure of our circRNA, which contains MS2 aptamer.The results are below:

Figure 1.2D stucture predicted by RNAfold

Figure 2.2D stucture predicted by StructureEditor

3D stucture

UNIQ83950070b5c06cdf-partinfo-00000003-QINU UNIQ83950070b5c06cdf-partinfo-00000004-QINU
We use this website:http://biophy.hust.edu.cn/new/3dRNA/create, to predict the 3D structure of our circRNA, which contains MS2 aptamer.The result lists below:

Figure 3. 3D stucture of MS2 aptamer predicted by Xiaolab

MS2 binding mechanism

UNIQ83950070b5c06cdf-partinfo-00000005-QINU UNIQ83950070b5c06cdf-partinfo-00000006-QINU

Figure 4. MS2 binding mechanism

split EGFP

UNIQ83950070b5c06cdf-partinfo-00000007-QINU UNIQ83950070b5c06cdf-partinfo-00000008-QINU
We are SYSU-CHINA,and this year we use this part as MS2 coat protein's aptamer to do a split EGFP experiment,for the proof of concept.Here we found a research article, which use liner RNA with MS2 and PP7 aptamer, to do the split EGFP experiment.Below are their experimental data.

Figure 3.the principle and experimental data of split EGFP

UNIQ83950070b5c06cdf-partinfo-00000009-QINU