Part:BBa_K3887008

LacO-scar-T7-LacO Promoter

This is an optimized part of [BBa_K2406020|BBa_K2406020]] .

Decriptioon

T7 promoter is commonly used in iGEM projects, [Part:BBa_K2406020|BBa_K2406020]] designed by iGEM17_Edinburgh_UG was an inducile T7-LacO promoter for gene expression. In our project, we also used T7-LacO promoter to control the expression of halogenase. However, we found high basic leakage of this promoter, which affected the results of our experiments, so we improved this promoter in our project. It is recognized that an excellent promoter should possess exceptional switch ratio: it should have lower background leakage in the "off" state and have obvious expression in the "on" state. In order to reduce the leakage and to ensure the repressor can bind better to the LacO site, we added another LacO site in the T7-LacO promoter as the figure below shows:

Fig.1 Improved promoter and original promoter

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]