DNA

Part:BBa_K3822003

Designed by: Jiajia Li   Group: iGEM21_YiYe-China   (2021-09-17)
Revision as of 05:16, 13 October 2021 by Ljj (Talk | contribs)


Hypermethylated region of TFPI2

The sequence of Hypermethylated region of TFPI2.We use this part for detection of colorectal cancer (CRC).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

In our project, the trigger sequence is linked to this part by DNA amplification and was added into the cell free system together with the plasmid contain toehold sequence, the report fluorescent protein will be expressed. The visible color and fluorescent signal can indicate the methylation degree of the TFPI2 gene in CRC patients.


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