Part:BBa_K3930023
Galactose inducible promoter
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 70
- 1000COMPATIBLE WITH RFC[1000]
The promoter Gal1 is an inducible promoter upon addition of galactose into the media of culture. This sequence comes from the Sacharomyces cerevisiae genome. This part is related to the extensively used and characterized part BBa_J63006. Nonetheless, our promoter lack the kozak sequence.
Introduction
The promoter Gal1 is an inducible promoter upon addition of galactose into the media of culture. This sequence comes from the Sacharomyces cerevisiae genome. This part is related to the extensively used and characterized part BBa_J63006. Nonetheless, our promoter lack the kozak sequence.
Results
Production of α-ionone (BBa_K3930003)
The part (BBa_K3930003) was linearized and transformed into the S.cerevisiae LycoYeast strain. The production of α-ionone was induced by plating the transformant onto a YPGal media (20mg.ml-1 of galactose). Figure 1 shows the mass spectra between a induced and a none induced strain.
This promoter gal without the kozak sequence work under those lab experimentations.
None |