Coding

Part:BBa_K3846003

Designed by: Marcel Arvid Zimmeck   Group: iGEM21_Hamburg   (2021-10-12)
Revision as of 15:45, 12 October 2021 by Registry (Talk | contribs)

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Amorpha-4,11-diene 12-monooxygenase (CYP71AV1)

Involved in the biosynthesis of the antimalarial endoperoxide artemisinin (PubMed:16458889, PubMed:16612385, PubMed:20351109, PubMed:27488942).

Catalyzes three consecutive oxidations of amorpha-4,11-diene to produce artemisinic acid, with artemisinic alcohol and artemisinic aldehyde as intermediates products, but is unable to oxidize germacrene A (PubMed:16458889, PubMed:16612385, PubMed:20351109, PubMed:23246612).

No activity with limonene, alpha-pinene, beta-pinene, pinocarveol, (-)-alloisolongifolene, caryophyllene, (-)-alpha-gurjunene, (+)-gamma-gurjunene, (+)-ledene, (+)-beta-selinene and (+)-valencene as substrates (PubMed:16458889, PubMed:16612385, PubMed:20351109).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None