Part:BBa_K3971011

cscB (sucrose permease) for sucrose transport.

Usage and Biology

CscB (sucrose permease) is a sucrose/H+ symporter from E. coli W that relies on an H+ gradient across the cell membrane to transport sucrose, and allows bacteria to take up sucrose [1]. The first study of using CscB as a sucrose exporter in cyanobacteria was reported by Ducat et. al [2] where they showed that Synechococcus elongatus 7942 could export sucrose irreversibly to concentrations of >10 mM without culture toxicity.

The higher pH value in the extracellular medium creates a reversed proton gradient, which enables the engineered cyanobacteria to export sucrose outside the cell [1].

csc operon in E.coli W for sucrose metabolism. Figure adapted from: Bruschi, Michele, et al. "A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains." Biotechnology advances 30.5 (2012): 1001-1010.

References

1: Lin, Po-Cheng, Fuzhong Zhang, and Himadri B. Pakrasi. "Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973." Scientific reports 10.1 (2020): 1-8.

2: Ducat, Daniel C., et al. "Rerouting carbon flux to enhance photosynthetic productivity." Applied and environmental microbiology 78.8 (2012): 2660-2668. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 481
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 525