Part:BBa_K3782001
T7-RBS-His-FfIBP-Term
FfIBP is a protein coding region that codes for an ice binding protein (IBP). IBPs, or more specifically antifreeze proteins (AFP), can bind to ice crystals and thereby prevent further ice growth. They are produced by organisms to survive in extremely cold environments. Activities of AFPs can be characterized by their thermal hysteresis (TH) or by their ice recrystallization inhibition (IRI). TH activity corresponds to the lowering of the freezing point without changing the melting point of a solution. IRI activity inhibits the growth of large ice crystals at the expense of smaller ones. The combination of these activities, which vary depending on the protein structure, prevents the freezing of body fluids and cell damage in organisms that live in environments with extremely cold temperatures. This part contains a T7 strong promoter, RBS, the Flavobacterium frigoris ice-binding protein (FfIBP) with a His tag at the N-terminus, followed by a terminator. The 6x His tag was added to purify the protein using a his tag affinity column.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 347
Illegal PstI site found at 428
Illegal PstI site found at 608 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 830
Illegal PstI site found at 347
Illegal PstI site found at 428
Illegal PstI site found at 608 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 347
Illegal PstI site found at 428
Illegal PstI site found at 608 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 347
Illegal PstI site found at 428
Illegal PstI site found at 608 - 1000COMPATIBLE WITH RFC[1000]
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