Translational_Unit

Part:BBa_K3745030:Design

Designed by: Jiaqi Tang   Group: iGEM21_BNDS_China   (2021-09-30)
Revision as of 12:35, 11 October 2021 by Jiaqi Tang (Talk | contribs)


Plac+rhlABC linear fragment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3524
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2149
    Illegal NgoMIV site found at 2458
    Illegal NgoMIV site found at 4067
    Illegal AgeI site found at 3070
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4206


Design Notes

It is worth informing that the Plac in the DNA feature's graph is a reverse promoter (activate LacI instead of activating rhlA, B, and C). The arrow on the feature is not accurate due to the reason that the image of the promoter won't appear in the reverse direction. Nevertheless, the DNA sequence is posted in the correct arrangement which Plac is responsible for activating LacI.


Source

This study

References