DNA

Part:BBa_K313010:Experience

Designed by: Ryosuke Kamei, Ryo Kariyazono, Takashi Matsumoto, Takashi Hiroi, Mei Kobayashi, Yuka Yashiro, Tomoka Ao   Group: iGEM10_UT-Tokyo   (2010-10-22)
Revision as of 09:49, 11 October 2021 by Teapot657 (Talk | contribs) (→‎3D stucture)


We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.

Applications of BBa_K313010

We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.

2D stucture

UNIQa22cf8035d4f4738-partinfo-00000000-QINU UNIQa22cf8035d4f4738-partinfo-00000001-QINU We use RNAfold and StructureEditor to predict 2D structure of our circRNA, which contains MS2 aptamer.The results are below:

MS2 aptamer on the circRNA scaffold.png
Figure 1.2D stucture predicted by RNAfold

SYSU-CHINA circRNA-1.png
Figure 2.2D stucture predicted by StructureEditor

3D stucture

UNIQa22cf8035d4f4738-partinfo-00000002-QINU UNIQa22cf8035d4f4738-partinfo-00000003-QINU We use this website:http://biophy.hust.edu.cn/new/3dRNA/create, to predict the 3D structure of our circRNA, which contains MS2 aptamer.The result lists below: