Coding

Part:BBa_K3895003

Designed by: Rui Zhu   Group: iGEM21_SZ_SHD   (2021-10-01)
Revision as of 15:27, 10 October 2021 by Auskuld (Talk | contribs)


Keratinase kerAvDZ50

KerAVDZ50 is an extracellular serine thiol alkaline protease from the Actinomadura viridilutea strain, which can be isolated from the Algerian fishing port [1]. As a thermo-alkaline keratinases, it can function at a pH range of 7–12 and a temperature range of 35–80 °C.

6x His-tags were added to both sides of the sequence for purification.

Modeling

Figure 1. Swiss Model showing serine residues of KerAVDZ50.
Figure 2. Swiss Model showing hydrophobisity of KerAVDZ50.
Figure 3. Ramachandran Plot of KerAVDZ50.Z50 Ramachandran Favoured is 95.14%


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 577
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 382
    Illegal AgeI site found at 1120
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 322


Reference

Biochemical and molecular characterization of new keratinoytic protease from Actinomadura viridilutea DZ50. (n.d.). International Journal of Biological Macromolecules, 92, 299–315. https://doi.org/10.1016/j.ijbiomac.2016.07.009

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Parameters
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