Part:BBa_K3487005
Non-specific endonuclease
Description
RalR acts as a non-specific endonuclease that can cleave methylated and unmethylated DNA, then leading to cell death. As a novel type I TA system, RalR is the first toxin that functions as an endonuclease in E. coli. Y.X. Guo,et al found that RalR in E. coli functions differently, acting as a toxin by cleaving DNA, and that it belongs to a type I toxin–antitoxin system. In our project, we used RalR as a toxin protein to cleave the DNA of host cell to avoid the bacterial escape.In our project,we used RalR as a toxin protein to cleave the DNA of host cell to avoid the bacterial escape. We test the function of this toxin protein in E. coli. The result is as follow. 11111
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
2020 SZPT-CHINA
Result
We streaked the engineered strain into a 1Mm IPTG plate for overnight culture, and observed that the engineered strain containing the RalR gene did not grow on the plate. The expression of RalR was good under the positive and negative controls, and the colony situation in the corresponding area was significantly less than that of the empty vector control group. The result is as follow.
References
Guo Y, Quiroga C, Chen Q, et al. RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Res. 2014;42(10):6448-6462.
| None |