Composite

Part:BBa_K3739106

Designed by: Shichen Geng   Group: iGEM21_XMU-China   (2021-09-30)
Revision as of 13:03, 10 October 2021 by Oliviatong (Talk | contribs) (more description)


K525998(T7-RBS)-Aly01-his-CBM-hutH-B0010

Aly01 here represents a signal peptide used to secrect the fusion protein outside the cell, his-tag enable us to purify the linking protein. The fusion protein CBM-hutH works on the surface of single cell Phaeocystis. globosa and the enzyme catalyzes the reaction of converting histidine to form toxic urocanic acid. His-tag is added to purify the protein. We use BBa_K3739106 to construct the expression system and to express and to purify the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 653
    Illegal NgoMIV site found at 1089
    Illegal NgoMIV site found at 1824
    Illegal NgoMIV site found at 2060
    Illegal AgeI site found at 111
    Illegal AgeI site found at 399
    Illegal AgeI site found at 489
    Illegal AgeI site found at 726
    Illegal AgeI site found at 1553
    Illegal AgeI site found at 2049
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None