Part:BBa_K3779009:Experience

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Applications of BBa_K3779009

① Codon optimization</br>   The original target gene BBa_K3779022 was codon optimized according to pichia pastoris preference, and the optimized codon SS-bgly (BBa_K3779001) was used for plasmid construction to improve the activity of our enzyme. ②the signal peptide</br>   We added the signal peptide α-factor (BBa_K3779002) to our plasmid, which contributed to the extracellular expression of our target protein in pichia pastoris. ③ AOX1 promoter</br>   We used methanol-induced promoters and designed 5 'and 3' primers BBa_K3779000 and BBa_K3779003. Integration of foreign genes ④ Integration of foreign genes</br>   Linearized plasmids were introduced into p. Pastoris genome for homologous integration by electrotransformation. This method is simple and efficient in practice.

  The recombinant expression strain of pichia pastoris β -glycosidase was constructed, as shown in figure. <img src="" style="width:200px;height:200px;"></a> Results As shown in the figure, there was a target band of about 2000 bp in line with the theoretical size, while the blank control GS115-pPIC9K only had a band of 500 bp, proving that the recombinant strain was successfully constructed. （3）链接

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