Plasmid

Part:BBa_K3779009:Experience

Designed by: JiaCheng, Li   Group: iGEM21_NWU-CHINA-B   (2021-06-21)
Revision as of 12:15, 10 October 2021 by JianMing-Fu (Talk | contribs) (→‎Applications of BBa_K3779009)


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Applications of BBa_K3779009

① Codon optimization The original target gene BBa_K3779022 was codon optimized according to pichia pastoris preference, and the optimized codon SS-bgly (BBa_K3779001) was used for plasmid construction to improve the activity of our enzyme. ②the signal peptide We added the signal peptide α-factor (BBa_K3779002) to our plasmid, which contributed to the extracellular expression of our target protein in pichia pastoris. ③ AOX1 promoter We used methanol-induced promoters and designed 5 'and 3' primers BBa_K3779000 and BBa_K3779003. Integration of foreign genes ④ Integration of foreign genes Linearized plasmids were introduced into p. Pastoris genome for homologous integration by electrotransformation. This method is simple and efficient in practice.

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