RBS

Part:BBa_K3740018

Designed by: Guiyi Huang   Group: iGEM21_SZPT-CHINA   (2021-08-26)
Revision as of 06:03, 10 October 2021 by HUANG (Talk | contribs) (→‎2021 SZPT-China)


RBS004

Description

Responsible for the recruitment of a ribosome during the initiation of translation

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



2021 SZPT-China

Biology

Mutagenesis at specific position of RBS (BBa_B0034) was achieved RBS004 (BBa_K3740018) using random primers and PCR, and the constructed plasmid J23100-RBS004-sfGFP-rrn B T1 (BBa_K3740059). Compare the fluorescence intensity of sfGFP induced by J23100-B0034-sfGFP-rrn B T1 (BBa_K3740058)


Usage

Constructed J23100-RBS004-sfGFP-rrn B T1 (BBa_K3740059) was transformed into E. coli DH5α. we quantified the fluorescence intensity when the optical absorbance of bacterial culture at 600nm was around 0.2

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Categories
Parameters
None