Part:BBa_K3838111

J-SHSP

SHSP is a stress protein and heat shock protein, which is a group of proteins synthesized preferentially by cells. Compared with the mutant strains lacking plasmid encoding SHSP, the parent strains carrying plasmid encoding SHSP were significantly more resistant to temperature changes from 42℃ to 62℃, and there was a significant difference in cell survival rate between SHSP plasmid carrying S. thermophilus and SHSP elimination plasmid under heat shock conditions. Transformation of cloned SHSP genes into streptococcus thermophilus strains lacking plasmid encoding SHSP resulted in increased resistance to culture at 60℃ or pH 3.5 and increased ability to grow at 52℃. Meanwhile, the presence of PET-SHSP and the overexpression of SHSP gene demonstrated its ability to protect E.coli cells from ethanol stress. The literature showed that escherichia coli cells overexpressing SHSP gene of the smaller plasmid pQC2 of STREptococcus thermophilus ST-QC significantly increased resistance to heat, acid and ethanol stress. Therefore, we chose HSP as a non-antibiotic screening method for E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 142
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Tian H, Tan J, Zhang L, et al. Increase of stress resistance in Lactococcus lactis via a novel food-grade vector expressing a shsp gene from Streptococcus thermophilus. Braz J Microbiol. 2012;43(3):1157-1164. doi:10.1590/S1517-838220120003000043