Composite

Part:BBa_K3755041

Designed by: Kaijun Wang   Group: iGEM21_ShanghaiTech_China   (2021-10-01)
Revision as of 15:34, 9 October 2021 by Arisaema (Talk | contribs) (→‎Usage and Biology)


CMV enhancer+CMV promoter+GCaMP6m+SV40 PolyA signal

This composite part is constructed to express GCaMP6m in mammalian cells.

Usage and Biology

This part is cloned in pEGFP, that's why CMV enhancer, CMV promoter and SV40 PolyA signal are used. GCaMP6m is expressed in this composte part. GCaMP6m is a genetically encoded calcium indicator. When intracellular calcium concentration increases, emitted light at about 510nm will increase obviously. To see more information about GCaMP6m, please to to view Part:BBa_K3755007. In our part, we used GCaMP6m to test whether calciums flow in cells after mechanical stimulation as we expected.

Experiment and result

Please go to BBa_K3755007 to view the experiments and results of GCaMP6m.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 542
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 683
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1524
    Illegal SapI.rc site found at 1430


[edit]
Categories
//awards/composite_part/nominee
Parameters
None