Reporter

Part:BBa_K3889002

Designed by: Prameya Garge, Ashwin Sharma   Group: iGEM21_IISER-Tirupati_India   (2021-10-02)
Revision as of 14:34, 9 October 2021 by AshWinShaRma (Talk | contribs)


modified_sfGFP

sfGFP is a variant of Green Fluorescence Proteinfrom Aequorea victoria and has the following parameters [1]:

Ex : Peak excitation wavelength (in nm) 485
Em: Peak emission wavelength (in nm) 510
EC: Extinction Coefficient (M-1 cm-1) 83,300
QY: Quantum Yield 0.65
Brightness 54.15
Maturation (min) 13.6


It can be used for reporting the expression of the desired promoter.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13



References

1. https://www.fpbase.org/protein/superfolder-gfp/

[edit]
Categories
Parameters
None