Part:BBa_I742107

COMT

COMT coding sequence

Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring, converting caffeic acid to ferulic acid) from Medicago sativa (alfalfa). This gene was synthesised by Geneart.

IISER_BHOPAL 2021

Usage and Biology

It has been found that strong downregulation of COMT in transgenic alfalfa plants decrease the lignin amount and a reduction in the number of units of guaiacyl (G) lignin with a total loss of syringyl (S) units in monomeric and dimeric lignin degradation products.[1]

References

1.Guo, D., Chen, F., Inoue, K., Blount, J. W., & Dixon, R. A. (2001). Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin. The Plant Cell, 13(1), 73. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 577