Part:BBa_K3782000

Flavobacterium frigoris strain PS1 ice-binding protein gene

FfIBP

FfIBP is a protein coding region that codes for an ice binding protein (IBP). IBPs, or more specifically antifreeze proteins (AFP), can bind to ice crystals and thereby prevent further ice growth. They are produced by organisms to survive in extremely cold environments. Activities of AFPs can be characterized by their thermal hysteresis (TH) or by their ice recrystallization inhibition (IRI). TH activity corresponds to the lowering of the freezing point without changing the melting point of a solution. IRI activity inhibits the growth of large ice crystals at the expense of smaller ones. The combination of these activities, which vary depending on the protein structure, prevents the freezing of body fluids and cell damage in organisms that live in environments with extremely cold temperatures.

Profile

Name	FfIBP
Base pairs	744
Number of amino acids	247
Molecular weight	25.35 kDa
Origin	Flavobacterium frigoris strain PS1, synthetic

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 269
  Illegal PstI site found at 350
  Illegal PstI site found at 530
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 269
  Illegal PstI site found at 350
  Illegal PstI site found at 530
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 269
  Illegal PstI site found at 350
  Illegal PstI site found at 530
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 269
  Illegal PstI site found at 350
  Illegal PstI site found at 530
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

FfIBP is naturally produced by the Gram-negative Antarctic bacterium Flavobacterium frigoris PS1. Its TH activity is around 2.5 K at 50 µM.