Plasmid

Part:BBa_K3755032:Design

Designed by: Qi Xin   Group: iGEM21_ShanghaiTech_China   (2021-10-01)
Revision as of 09:15, 9 October 2021 by HeteroauXin (Talk | contribs) (→‎Source)

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pAAV.Syn.Flex.GCaMP6m.WPRE.SV40


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3444
    Illegal EcoRI site found at 5135
    Illegal PstI site found at 2973
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3444
    Illegal EcoRI site found at 5135
    Illegal NheI site found at 3455
    Illegal NheI site found at 3644
    Illegal PstI site found at 2973
    Illegal NotI site found at 5773
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3444
    Illegal EcoRI site found at 5135
    Illegal BglII site found at 2967
    Illegal BglII site found at 5066
    Illegal BamHI site found at 3463
    Illegal BamHI site found at 4970
    Illegal XhoI site found at 3785
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3444
    Illegal EcoRI site found at 5135
    Illegal PstI site found at 2973
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3444
    Illegal EcoRI site found at 5135
    Illegal PstI site found at 2973
    Illegal NgoMIV site found at 2581
    Illegal NgoMIV site found at 3301
    Illegal NgoMIV site found at 3373
    Illegal NgoMIV site found at 5671
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4626
    Illegal BsaI.rc site found at 1405
    Illegal SapI site found at 322
    Illegal SapI.rc site found at 4532


Design Notes

This is the plasmid which is our PCR template, so we have not design note.

Source

This is the plasmid which is our PCR template, so we have not source information.

References