Part:BBa_K3896005

PmrCAB(DPP4)-LuxI-LuxR

We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original PmrB with the core binding domain of DPP4 protein to be stimulated by the S protein of MERS-CoV. After detecting the S protein of MERS-CoV, the sensor kinase PmrB autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA. Then phosphorylated PmrA protein combined with promoter PmrC sequence to activate the expression of reporter gene.

The downstream genes will express AHL and LuxR, mediating the quorum sensing system. In the AHL mediated quorum sensing system composed of LuxI/LuxR, LuxI homologous gene in bacteria is responsible for the synthesis of QS signal molecule AHL, and LuxR homologue is activated as the receptor of this signal molecule to regulate the transcription of downstream genes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1532
  Illegal NheI site found at 4479
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 637
  Illegal BamHI site found at 4907
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2517
  Illegal AgeI site found at 3726
  
• 1000
  COMPATIBLE WITH RFC[1000]