Part:BBa_K3896017
Plux_HS-LuxI-EGFP
After reaching the threshold, AHL can combine with endogenous LuxR to form a complex and activate the corresponding promoter PluxR. In this project, we selected the improved promoter Plux_HS of igem2018 Tsinghua University team!!!!!, the promoter has the advantage of low leakage.After Plux_ HS is activated, the expressed AHL will combine with endogenous LuxR to form a complex and furtheriy activate Plux_ HS promoter, so as to achieve the effect of cascade amplification, and finally express a large number of EGFP, making the detection effect significant.
We first verify the above circuits by artificially adding AHL.
figure (contrast of AHL and none)
We want to know the concentration of AHL and set a series of gradients. The results are as follows:
figure (result) Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 721
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1400
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