Regulatory

Part:BBa_K3726078:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-08)
Revision as of 16:19, 8 October 2021 by Sbarraga (Talk | contribs)


P_Pgntk


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.

Source

The sequence of this part belongs to Escherichia coli BW25113 genome.

References

S. Boyarskiy, S. Davis LĂłpez, N. Kong and D. Tullman-Ercek, "Transcriptional feedback regulation of efflux protein expression for increased tolerance to and production of n-butanol", 2021.