Part:BBa_K3726107
3CON5(H)_nNS2-down (PCC 11801)
This part is an homology 3’ connector harboring the down homology arm for the homologous recombination within the Neutral Site Nº 2 of PCC11801.
Within the Marburg Collection design, connectors are found in the upstream (5’) and downstream (3’) regions of the level 1 assemblies, flanking the transcriptional unit. This allows the further assembly of multiple transcriptional units into a level 2 genetic construct using level 1 parts flanked by different connectors. To know more about, read the design principles of Marburg Collection: https://2019.igem.org/Team:Marburg/Part_Collection.
Among other functions, connectors can also be used to include homology regions for integrative recombination in the chassis genome. This way 3’ Connector parts can harbor the up / down homology arm required for double homologous recombination.
The part has been designed in accordance with the Marburg Collection guidelines. 5’ / 3’ Connectors correspond with the parts found Upstream / Downstream of the level 1 assembled transcriptional unit.
Sequence & Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 646
Illegal PstI site found at 125 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 646
Illegal PstI site found at 125 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 646
Illegal XhoI site found at 371
Illegal XhoI site found at 386 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 646
Illegal PstI site found at 125 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 646
Illegal PstI site found at 125
Illegal NgoMIV site found at 396 - 1000COMPATIBLE WITH RFC[1000]
The genomic integration loci targeted by this part corresponds with a novel neutral site for Synechococcus PCC11801. This neutral site has been identified by the MADRID_UCM 2021 iGEM team as a part of their project. To know more about the discovery process visit the section Software of 4C_Fuels project. https://2021.igem.org/Team:MADRID_UCM/Software
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