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Part:BBa_K3726104:Design

Designed by: Lucia Tapia   Group: iGEM21_MADRID_UCM   (2021-10-06)
Revision as of 14:09, 8 October 2021 by LuciaTapia (Talk | contribs)


5CON1(H)_NS1(mod)-up (PCC 11801)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 18
    Illegal AgeI site found at 594
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a 5’CON part the upstream overhang is AACA , while the downstream overhang is GGAG.


Source

Homology conncetor has been found within the genome of Synechococcus elongatus PCC 11801.