Coding

Part:BBa_K3970003

Designed by: Xuan Gong   Group: iGEM21_ECUST_China   (2021-10-01)
Revision as of 12:29, 8 October 2021 by MenghuaLi (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pLEU2-LEU2

Usage and Biology

In yeast, the LEU2 gene encodes a β-propyl malate dehydrogenase, which catalyzes the third step of leucine biosynthesis, the conversion from β-isopropyl malate to α-ketoishaproic acid. Because β-isopropyl malate dehydrogenase (EC 1.1.1.85) is quite conserved in its regulatory mechanism and primary structure, LEU2 gene is a useful selection marker in a variety of yeast.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None