Coding

Part:BBa_K3930017

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-08)
Revision as of 07:15, 8 October 2021 by Registry (Talk | contribs)

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Fusion between phCCD1 and the fyn anchor with linker to produce β-ionone

This sequence codes for a phCCD1 that transforms beta-carotene into beta-ionone, fused a fyn anchor, which allows relocation to the membrane of phCCD1. These sequence is codon optimized for an expression into S.cerevisiae. The phCCD1 sequence comes from Petunia hybrida.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 864
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None