Coding

Part:BBa_K3930016

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-08)
Revision as of 07:05, 8 October 2021 by Registry (Talk | contribs)

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Fusion between CrtY and phCCD1 with a LGS linker to produce β-ionone

This sequence codes for an enzymayivc fusion between CrtY that transforms Lycopene into beta-carotene, and phCCD1 that transforms beta-carotene into beta-ionone. Those two sequences are codon optimized for an expression into S.cerevisiae. The CrtY sequence comes from Pantoea ananatis and phCCD1 comes from Petunia hybrida.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1641
    Illegal BglII site found at 1824
    Illegal BamHI site found at 1989
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None