Coding

Part:BBa_K3853003

Designed by: Gongyu Liu   Group: iGEM21_CPU_CHINA   (2021-09-20)
Revision as of 21:30, 7 October 2021 by Lp-tiffany (Talk | contribs)


dCas9

For dCas9, two nuclease splicing active domains of CRISPR associated protein 9 (Cas9), RuvC and HNH, are mutated simultaneously (D10A & H840A; RuvC- & HNH-) to obtain a protein that has no nuclease activity. The dCas9 protein has no ability to cut DNA, but can still bind to specific DNA sequences under the single-gudie RNA. The codons of the protein gene sequence are optimized to increase its expression in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3960
    Illegal AgeI site found at 3304
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None