Coding

Part:BBa_K3809010:Design

Designed by: Francisco Javier Castañeda Villagran   Group: iGEM21_TecCEM   (2021-10-07)
Revision as of 19:01, 7 October 2021 by Registry (Talk | contribs)

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ESR1 with Linker, His Tag and Signal Peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1264


Design Notes

In order to express the protein in a bacterial system, we optimized the codons for E. coli using Benchling.


Source

The Signal Peptide sequence NPS4 was obtained from BBa_K3606042, the linker was a modified version of BBa_K1486004, with the addition of a cistein; ESR1 sequence was obtained from Homo sapiens estrogen receptor 1 (ESR1), transcript variant 1, mRNA, NCBI Reference Sequence: NM_000125.4.

References