Terminator

Part:BBa_K3726095:Design

Designed by: Lucia Tapia   Group: iGEM21_MADRID_UCM   (2021-10-06)
Revision as of 17:26, 7 October 2021 by Fmjorge99 (Talk | contribs)

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T(5a)_Teck


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.

This terminator part spans the 5A position of the MoClo syntax. The downstream overhang is GGTA, allowing the assembly of an additional expression cassette downstream the terminator part.


Source

This part has been artificially designed and synthetized as part of the research of G. Gale, B. Wang and A. McCormick.

References

G. Gale, B. Wang and A. McCormick, "Evaluation and Comparison of the Efficiency of Transcription Terminators in Different Cyanobacterial Species", Frontiers in Microbiology, vol. 11, 2021.