Part:BBa_K3726104

5CON1(H)_NS1(mod)-up (PCC 11801)

This part is an homology 5’ connector harboring the up homology arm for the homologous recombination within the Neutral Site Nº 1 of PCC11801.

Within the Marburg Collection design, connectors are found in the upstream (5’) and downstream (3’) regions of the level 1 assemblies, flanking the transcriptional unit. This allows the further assembly of multiple transcriptional units into a level 2 genetic construct using level 1 parts flanked by different connectors. To know more about, read the design principles of Marburg Collection. (Referencia a Marburg).

Among other functions, connectors can also be used to include homology regions for integrative recombination in the chassis genome. This way 5’ Connector / 3’ Connector parts can harbor the up / down homology arm required for double homologous recombination.

The part has been designed in accordance with the Marburg Collection guidelines. 5’ / 3’ Connectors correspond with the parts found Upstream / Downstream of the level 1 assembled transcriptional unit.

Sequence & Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 18
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 18
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 18
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 18
  Illegal AgeI site found at 594
  
• 1000
  COMPATIBLE WITH RFC[1000]