Part:BBa_K3732000

photosensitive degron (psd) module.

The psd module can be attached to the carboxy terminus of target proteins that are localized to the cytosol or nucleus to obtain light control over their stability. Blue light induces structural changes in the LOV2 domain, which in turn lead to activation of the degron and thus proteasomal degradation of the whole fusion protein. Variants of the psd module with diverse characteristics are useful to fi ne-tune the stability of a selected target at permissive (darkness) and restrictive conditions (blue light).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Contribution from iGEM21-FAFU-China

LOV2

The light-receptor domain LOV2 binds flavin mononucleotide (FMN) as a cofactor, which is the main photoactivated compound. Excitation of the FMN cofactors by blue light results in the formation of a bond between FMN and cysteine in the LOV2 domain. This induces structural rearrangement of the LOV2 domain, leading to the unfolding of the α -helix at the carboxyl terminal. ^「1」

cODC1

In ODC, the carboxy-terminal 37 amino acids (cODC) function as a degron that is directly recognized by the proteasome. ^「2」

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When LOV2 is activated by light，which makes the cODC1 degron accessible for recognition by the proteasome and ubiquitin - independent degradation of the whole construct.^「1」