DNA

Part:BBa_K3905018

Designed by: Peter Heywood   Group: iGEM21_City_of_London_UK   (2021-10-07)
Revision as of 12:12, 7 October 2021 by Registry (Talk | contribs)

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517-5 T7p* Probe 2

Amplification probe required for the isothermal amplification of miRNA using Recombinase polymerase amplification. RPA of the miRNA required the design of the 517-5 T7p* Probe 2. Probe 2 binds to the 3' end of the miRNA. After phosphorylation of 517-5 T7p* Probe 1, DNA ligase is able to ligate 517-5 T7p* Probe 2 to 517-5 T7p* Probe 1. Allowing RPA to occur for the formation of DNA. For this to occur, the reverse primer 5' AGTGGAGGGTGGAGGGAGGTGGAGGG 3' is added which binds to this probe.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None